Differential Modulation of Heat-Inducible Genes Across Diverse Genotypes and Molecular Cloning of a sHSP From Pearl Millet [Pennisetum glaucum (L.) R. Br.]

The survival, biomass, and grain yield of most the crops are negatively influenced by several environmental stresses. present study was carried out using transcript expression profiling for functionally clarifying role genes belonging to a small heat shock protein (sHSP) family in pearl millet under high-temperature stress. Transcript two high-temperature-responsive marker genes, Pgcp70 PgHSF , along with physio-biochemical traits considered screen best contrasting genotypes among eight different inbred lines seedling stage. pattern suggested existence differential response upon stress form accumulation shock-responsive gene transcripts. Genotypes, such as WGI 126, TT-1, TT-6, MS 841B, responded positively toward both also indicated better growth PPMI-69 showed least responsiveness induction; moreover, it supports membrane stability index (MSI) data scoring thermotolerance, thereby suggesting efficacy molecular-based screening technique identification thermotolerant at particular crop stages. genotypes, (thermosusceptible) WGI-126 TT-1 (thermotolerant), further utilized characterization thermotolerance behavior sHSP cloning PgHSP16.97 from cv. WGI-126. In addition, investigation extended 28 HSP20 through genome-wide search genome an understanding their RNA-sequencing (RNA-Seq) set. outcome that can be very useful high-throughput heat-tolerant Also, identified PgHSP20s provide insights into molecular regulation tolerance, bridging them together fight against unpredicted nature abiotic